成像设备

Axioplan 2. 蔡司Axioplan 2: The Axioplan 2 imaging is a universal upright microscope which is used for light (transmitted and incident) and fluorescence microscopy. Fluorescence illumination is provided by a Lumencor SOLA light engine. This light engine is more user and environmentally friendly by eliminating the need for a mercury lamp. The microscope is controlled by Micromanager software (operating a cooled ccd black/white Hamamatsu camera) and allows collection of time-lapse images in both DIC and fluorescent channels (DAPI, FITC, TRITC). An objective heater ensures tissue culture cell integrity during time-lapse experiments. 除了, the microscope is equipped with a digital color camera (SPOT, Insight) enabling collection of stained histology tissue sections.

Axioimager Z2. This is an upright microscope outfitted for both transmitted (including DIC, 和相位)和荧光成像. Fluorescence illumination is provided by an EXFO LED light source eliminating the mercury lamp. It is fully automated allowing acquisition in multiple X, Y and Z positions. 显微镜装有5倍至100倍的物镜. Image capture and analysis is through the Zeiss ZEN software. For large data sets there is an off-line image analysis workstation running ZEN.

徕卡星际5. 徕卡星际5 laser scanning confocal microscope: This system is ideal for 3D imaging of UV and visible fluorophores (4 colors) in fixed or live samples. This system combines transmitted light techniques with confocal imaging for a highly flexible platform. The Leica software running this system allows for time lapse, Z栈, 大面积瓷砖, 和三维重建.  For better than standard resolution the system is also equipped with Leica’s Lightning module.

蔡司LSM 880-AS快速Elyra.S1:  This system combines several technologies in to one flexible platform.  At the base level the system is the latest confocal microscope from Zeiss capable of imaging samples at multiple wavelengths from 405nm to 633nm and from magnifications of 10x to 100x.  Included on the system are two “super-resolution” modalities – the Airyscan/FAST unit and the Elyra unit.  The Airyscan technology improves resolution down to 140 nm laterally and 400 nm axially.   除了 the Airyscan module can be run in the “FAST” mode for live samples.  For users requiring better resolution than the Airyscan can provide the system has the Elyra unit which can provide resolution of 120nm laterally and 300nm axially.  Depending on experimental needs users can freely change between any of these technologies.  For samples requiring temperature control the system is outfitted with both heating and cooling inserts.

evo. 生命技术EVOS FL: This is an inverted microscope that allows imaging in a variety of configurations from slides to 96-well plates. The microscope is equipped with a variety of dry objectives and can be upgraded easily to include oil immersion objectives. Illumination is all LED eliminating the mercury lamp traditionally used for fluorescence illumination.

旋转的磁盘. 3i (Intelligent Imaging Innovations) spinning disk confocal: The facility houses an Intelligent Imaging Innovations (3I) integrated Yokogawa spinning disk confocal on a Zeiss Axio Observer fully automated inverted microscope.  旋转盘系统包括:4条激光线, 一个Prime 95B sCMOS相机(测光学), 温控舞台适配器(Okolab), the Vector2 system (3I) for photokinetic experiments and the Ablate module (3I) for targeted wounding in cells and tissues.

蔡司Axio变焦V16.  这是一台16倍变焦范围的立体显微镜.  It is equipped with both a color camera for transmitted/reflected light imaging and a monochrome camera for fluorescence imaging with 4 fluorescent cubes (DAPI, 绿色荧光蛋白, RFP和CY5).  此外，显微镜配备了Apotome.3 .使用荧光时进行光学切片.  It is fully motorized allowing acquisition of a large Z range.  Image capture and analysis is through the Zeiss ZEN software.  This microscope is ideally suited for imaging large specimens at high resolution.  For large data sets there is an off-line image analysis workstation running ZEN.